Applied Biosystems 7300 - Page 142

Appendix A
Moving the 7300/7500/7500 Fast System
116 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
5. Run a TaqMan
®
RNase P Instrument Verification
plate (see page 87).
• If the run passes, recalibrations are not
necessary.
• If the run fails, perform steps steps 6
through 9 to recalibrate the instrument.
6. Perform an ROI calibration (see page 35).
7. Perform a Background calibration (see page 49).
Perform an optical calibration if you are using a
7500 or 7500 Fast system (see page 59).
8. Perform a pure dye calibration (see page 67).
9. Perform an instrument verification run (see
page 87).