154 BD LSR II User’s Guide
Standard (4-Blue 2-Violet 2-UV 2-Red)
Configuration Specification
The standard configuration supports a blue octagon, and violet, UV, and red
trigons. Table E-1 shows the detectors, filters, and mirrors used in the standard
default configuration, and recommended fluorochromes per detector. The word
“blank” indicates that a blank optical holder should be used instead of a mirror
or filter. A dash (—) indicates that no slot exists for a mirror in that PMT
position.
Table E-1 Default filters and fluorochromes
Detector Array
(Laser)
PMT
(Detector)
Longpass
Dichroic
Mirror
Bandpass
Filter
Fluorochrome or
Scatter Parameter
blue octagon
(488-nm laser)
A 735 LP 780/60 PE-Cy7
B 685 LP 695/40 PerCP-Cy5.5
C 550 LP 575/26 PE, PI
D 505 LP 530/30 FITC, GFP
E blank 488/10 SSC
F blank blank none
G blank blank none
H — blank none
violet trigon
(405-nm laser)
A 505 LP 525/50 AmCyan
B blank 440/40 Pacific Blue™
C — blank none
UV trigon
(355-nm laser)
A 505 LP 530/30 Indo-1 (Blue)
B blank 450/50 Indo-1 (Violet), DAPI
C — blank none
LSR2.book Page 154 Tuesday, April 25, 2006 3:34 PM
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