Chapter 1: Introduction 29
• PRIME prepares the fluidics system by draining and filling the flow cell
with sheath fluid.
The fluid flow initially stops and pressure is reversed to force fluid out of
the flow cell and into the waste container. After a preset time, the flow cell
fills with sheath fluid at a controlled rate to prevent bubble formation or
entrapment. At completion, the instrument switches to STNDBY mode.
Sample Injection Port
The sample injection port (SIP) is where the sample tube is installed. The SIP
includes the sample injection tube and the tube support arm. Samples are
introduced through a stainless steel injection tube equipped with an outer droplet
containment sleeve. The sleeve works in conjunction with a vacuum pump to
eliminate droplet formation of sheath fluid as it backflushes from the sample
injection tube.
Figure 1-3 Sample injection port (SIP)
tube stop
Bal seal
outer sleeve
tube support arm
sample injection tube
LSR2.book Page 29 Tuesday, April 25, 2006 3:34 PM
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