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BD LSR II - Preparing the Fluidics System

BD LSR II
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Chapter 2: Instrument Setup 47
3 Add approximately 1 L of bleach to the waste container and close it.
4 Reconnect the orange waste tubing and make sure it is not kinked.
5 Reconnect the level sensor line.
Preparing the Fluidics
Next, make sure the fluidics system is ready. This section covers the following
preparations:
Removing Air Bubbles (below)
Priming the Fluidics on page 49
Removing Air Bubbles
Trapped bubbles can occasionally dislodge and pass through the flow cell,
resulting in inaccurate data.
NOTICE Do not vigorously shake, bend, or rattle the sheath filter or you might
damage it.
1 Check the sheath filter for trapped air bubbles.
2 If bubbles are visible, gently tap the filter body with your fingers to
dislodge the bubbles and force them to the top.
LSR2.book Page 47 Tuesday, April 25, 2006 3:34 PM

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