Chapter 5: Calcium Flux 89
Calcium Flux Optimization
Before beginning this section, do the following:
• Start up the instrument and perform QC.
• Ensure that the appropriate filters are installed. See Setting Up the Optical
Filters and Mirrors on page 39.
• Review the following section, Using the Time Parameter.
; Tip For calcium-flux experiments, use a sheath fluid that does not contain
preservatives. For example, use 1X phosphate-buffered saline (PBS).
Using the Time Parameter
The Time parameter shows how events change over time. In calcium flux
experiments, the Time parameter displays the rate at which the cells in the sample
respond to a stimulus.
The Time parameter is a fixed scale and cannot be altered. The values for the
Time parameter are in 10-ms increments; thus for a given event, a Time
parameter of 123 represents 1,230 ms. Because a plot spans 2.6–262,143,
tickmarks will span 2.6–2,621,430 ms (or 44 minutes). Therefore, it takes an
event approximately 44 minutes to travel from one end of a plot to the other.
; Tip Do not restart data recording during a calcium flux experiment; data will
be lost.
LSR2.book Page 89 Tuesday, April 25, 2006 3:34 PM
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