100 BD LSR II User’s Guide
The BD LSR II instrument is designed to require minimum maintenance.
However, to preserve the reliability of the instrument, you must regularly
perform basic preventive maintenance procedures. This chapter explains routine
cleaning procedures that will keep your instrument in good condition.
; Tip A 5% solution of sodium hypochlorite can be substituted for undiluted
bleach in the following cleaning procedures. However, higher concentrations of
sodium hypochlorite and use of other cleaning agents might damage the
instrument.
Daily Cleaning and Shutdown
Perform the following maintenance procedures every day:
• Daily Fluidics Cleaning on page 100
• Daily Shutdown on page 102
Daily Fluidics Cleaning
Each time you shut down the instrument, clean the sample injection tube and the
area between the injection tube and the outer sleeve (Figure 6-1 on page 101).
This prevents the sample injection tube from becoming clogged and removes dyes
that can remain in the tubing.
; Tip Follow this procedure immediately after running viscous samples or nucleic
acid dyes such as Hoechst, DAPI, propidium iodide (PI), acridine orange (AO), or
thiazole orange (TO).
All biological specimens and materials coming into contact with them are
considered biohazardous. Handle as if capable of transmitting infection.
Dispose of waste using proper precautions and in accordance with local
regulations. Never pipette by mouth. Wear suitable protective clothing,
eyewear, and gloves.
LSR2.book Page 100 Tuesday, April 25, 2006 3:34 PM
To Next Page
Loading...
