90 BD LSR II User’s Guide
Setting Up the Experiment
1 Choose Instrument > Instrument Configuration and verify the current
configuration.
Figure 3-1 on page 54 shows an Instrument Configuration dialog whose
current configuration is the standard default configuration.
Verify that Indo-1 (Violet) and Indo-1 (Blue) appear in the current
configuration.
For accurate data results, the Instrument Configuration dialog must reflect
the physical layout of the BD LSR II octagons and trigons. Modifications to
the current configuration will not apply unless you click Set Configuration.
2 Choose Edit > User Preferences.
Verify that under the General tab, all checkboxes are deselected (Figure 3-2
on page 55).
Refer to the BD FACSDiva Software Reference Manual for more
information about the instrument configuration and user preferences.
3 Create a new experiment and rename it CalciumFlux.
4 With the CalciumFlux experiment icon selected, verify in the Inspector that
the Use global instrument settings checkbox is checked (Figure 3-3 on
page 56).
LSR2.book Page 90 Tuesday, April 25, 2006 3:34 PM
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