BD LSR II - Lasers

Chapter 1: Introduction 33
Lasers
The BD LSR II flow cytometer has a fixed-alignment 488-nm laser with the
option of additional fixed-alignment lasers.
The primary blue 488-nm laser (Coherent Sapphire) generates forward scatter
(FSC) and side scatter (SSC) signals and four fluorescence signals.
• The optional red 633-nm laser (JDS Uniphase
1344P) generates two
fluorescence signals.
• The optional violet 405-nm laser (Coherent Radius 405) generates two
fluorescence signals.
• The optional UV 355-nm laser (Lightwave Xcyte) generates two
fluorescence signals.
Table 1-1 BD LSR II flow cytometer laser options
Laser Type
Wavelength
(Color)
Power
(mW)
Warm-Up
Time
(min)
Standard Coherent Sapphire™ solid state 488 nm (blue) 20 30
Optional JDS Uniphase 1344P helium-
neon
(HeNe) gas
633 nm (red) 17 20
Coherent Radius™ 405 solid state 405 nm (violet) 25 15
Lightwave Xcyte™ solid state 355 nm (UV) 20 30
LSR2.book Page 33 Tuesday, April 25, 2006 3:34 PM