Chapter 3: Running Samples 65
Recording and Analyzing Data
This section outlines some basic acquisition and analysis tasks using
BD FACSDiva software. The example shows data from two 4-color bead samples
with the following fluorochromes:
•FITC
•PE
•PerCP
•APC
The procedure builds on the results obtained in the previous exercise: Sample
Optimization Using Instrument Setup on page 52. To perform this procedure,
prepare two tubes containing all four fluorochromes.
If you use a different sample type (or if you have skipped the optimization
exercise), your software window content and your data plots and statistics may
deviate from those shown here. You might also need to modify some of the
instructions in the procedure.
For additional details on completing some of the steps below, refer to the
BD FACSDiva Software Reference Manual.
Preparing the Workspace
In this section, you prepare your workspace before recording data.
1 Using the Browser toolbar, create a new specimen in MyExperiment and
rename it FourColorBeads.
2 Create two tubes for the FourColorBeads specimen. Rename the tubes
Beads_001 and Beads_002.
3 Expand the Global Worksheets folder in MyExperiment to access the
default global worksheet, and rename the worksheet MyData.
LSR2.book Page 65 Tuesday, April 25, 2006 3:34 PM
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