86 BD LSR II User’s Guide
8 Draw a gate around the singlet CTN events.
9 Adjust the event rate to approximately 200 events/second with the
SAMPLE FINE ADJ knob.
10 Click Record Data to save the data.
11 Print the worksheet.
12 Remove the CTN tube from the cytometer and put the instrument in
standby mode.
Optimization for Data Recording
Optimize the instrument settings for the actual sample.
1 Install the sample tube and adjust the FSC and SSC voltages to place the
data events on scale.
2 Adjust the DAPI voltage to place the singlet diploid events at about channel
50 x 10
3
.
After optimizing the instrument settings, record data for each sample tube.
BD FACSDiva software does not include DNA analysis algorithms. Export your
data files for analysis in a third-party application such as ModFit LT™.
LSR2.book Page 86 Tuesday, April 25, 2006 3:34 PM
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