172 BD LSR II User’s Guide
6-Blue 6-Violet 0-UV 3-Red Configuration
6-Blue 6-Violet 0-UV 3-Red supports blue violet octagons, and a red trigon.
Table F-5 on page 173 shows the detectors, filters, and mirrors used in the
default configuration. The word “blank” indicates that a blank optical holder
should be used instead of a mirror or filter. A dash (—) indicates that no slot
exists for a mirror in that PMT position.
The 6-Blue 6-Violet 0-UV 3-Red maps are:
• 6-Color Blue Octagon Default Configuration Map on page 181
• 6-Color Violet Octagon Default Configuration Map on page 183
• 3-Color Red Trigon Default Configuration Map on page 185
violet trigon
(405-nm laser)
A 505 LP 525/50 AmCyan
B blank 450/50 Pacific Blue™
C — blank none
UV trigon
(355-nm laser)
A 505 LP 530/30 Indo-1 (Blue)
B blank 450/50 Indo-1 (Violet), DAPI
C — blank none
red trigon
(633-nm laser)
A 755 LP 780/60 APC-Cy7
B 710 LP 730/45 Alexa Fluor® 700
C — 660/20 APC
Table F-4 6-Blue 2-Violet 2-UV 3-Red default mirror and filter configuration (continued)
Detector Array
(Laser)
PMT
(Detector)
Longpass
Dichroic
Mirror
Bandpass
Filter
Fluorochrome or
Scatter Parameter
LSR2.book Page 172 Tuesday, April 25, 2006 3:34 PM
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