Chapter 2: Instrument Setup 49
Priming the Fluidics
Sometimes, air bubbles and debris are stuck in the flow cell. This is indicated by
excessive noise in the forward scatter parameter. In these cases, it is necessary to
prime the fluidics system.
1 Remove the tube from the SIP.
2 Press the PRIME fluid control button to force the fluid out of the flow cell
and into the waste container.
Once drained, the flow cell automatically fills with sheath fluid at a
controlled rate to prevent bubble formation or entrapment. The STNDBY
button is amber after completion.
3 Install a 12 x 75-mm tube with 1 mL of DI water on the SIP and place the
support arm under the tube.
4 Repeat the priming procedure, if needed.
Quality Control
An instrument quality control (QC) procedure, performed on a regular basis,
provides a standard for monitoring instrument performance. Instrument QC
consists of running QC samples and recording parameter means and CVs.
QC data should be analyzed for trends involving 10 or more runs. By keeping the
instrument settings and QC sample constant, changes in the recorded means and
CVs will indicate how much instrument performance varies over time.
QC results are affected by laser and fluidics performance. We strongly
recommend following the laser and fluidics maintenance procedures in
Chapter 6.
See QC Particles on page 150 for a list of acceptable QC beads.
A sample QC log is provided in Appendix B. It can be photocopied or used as a
guide in designing your own QC log.
LSR2.book Page 49 Tuesday, April 25, 2006 3:34 PM
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