28 BD LSR II User’s Guide
Fluidics
The purpose of the fluidics system is to carry the sample out of the sample tube
and into the sensing region of the flow cell. Cells are carried in the sample core
stream in single file and measured individually.
Sample Flow Rate Control
Three flow rate control buttons—LO, MED, and HI—set the sample flow rate
through the flow cell. The SAMPLE FINE ADJ knob allows you to adjust the
rate to intermediate levels.
When the SAMPLE FINE ADJ knob is at its midpoint, the sample flow rates at
the LO, MED, and HI settings are approximately 12, 35, and 60 µL/min of
sample, respectively. The knob turns five full revolutions in either direction from
its midpoint, providing sample flow rates from 0.5–2X the midpoint value. For
example, if the LO button is pressed, the knob will give flow rates from
approximately 6–24 µL/min.
Fluid Control
Three fluid control buttons—RUN, STNDBY, and PRIME—set the instrument
mode (Figure 1-2 on page 27).
• RUN pressurizes the sample tube to transport the sample through the
sample injection tube and into the flow cell.
The RUN button is green when the sample tube is on and the support arm
is centered. When the tube support arm is moved left or right to remove a
sample tube, the instrument switches to an automatic standby status to
conserve sheath fluid—the RUN button changes to orange.
• STNDBY (standby) stops fluid flow to conserve sheath fluid.
When you leave the instrument for more than a few minutes, place a tube
containing 1 mL of deionized (DI) water on the sample injection port (SIP)
and press STNDBY.
LSR2.book Page 28 Tuesday, April 25, 2006 3:34 PM
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