52 BD LSR II User’s Guide
Before You Begin
Before attempting to perform the procedures in this chapter, you should be
familiar with:
• BD LSR II instrument startup, setup, and QC procedures (see Chapter 2,
Instrument Setup on page 37)
• BD FACSDiva software concepts: workspace components, instrument and
acquisition controls, tools for data analysis.
To become familiar with BD FACSDiva software, perform the tutorial
exercises in Getting Started with BD FACSDiva Software.
For additional details, refer to the BD FACSDiva Software Reference
Manual.
Sample Optimization Using Instrument Setup
Before you record data for a sample, optimize the following for the sample type
and fluorochromes used:
• PMT voltages
• threshold setting
• compensation values
This section describes how to optimize samples using the Instrument Setup
feature of BD FACSDiva software. In particular, instrument setup automatically
calculates compensation settings. If you choose to perform compensation
manually, not all of the instructions given below will apply. For detailed
instructions, refer to the BD FACSDiva Software Reference Manual.
LSR2.book Page 52 Tuesday, April 25, 2006 3:34 PM
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