Chapter 3: Running Samples 53
Sample optimization consists of several main steps, to be performed in the order
listed below. Each of the steps is explained in greater detail in the sections that
follow.
1 Verify instrument configuration and user preferences.
2 Create an experiment.
3 Adjust voltages and the threshold setting.
4 Record the compensation tubes.
5 Calculate compensation settings.
The data shown in this example is from a 4-color bead sample with the following
fluorochromes:
•FITC
•PE
•PerCP
•APC
To perform the example exercise shown here, prepare an unstained control tube
and single-stained tubes for each fluorochrome.
If you follow this procedure with a different bead sample (or another sample
type), your software views, data plots, and statistics might deviate from those
shown here. Additionally, you might need to modify some of the instructions in
the procedure.
LSR2.book Page 53 Tuesday, April 25, 2006 3:34 PM
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