Chapter 6: Maintenance 103
Scheduled Maintenance
Perform the following maintenance procedures every 2 weeks:
• System Flush on page 103
• Waste Management System Maintenance on page 105
System Flush
An overall fluidics cleaning is required to remove debris and contaminants from
the sheath tubing, waste tubing, and flow cell. Perform the system flush at least
every 2 weeks.
1 Remove the sheath filter.
• Press the quick-disconnects on both sides of the filter assembly.
• Remove the filter assembly.
• Connect the two fluid lines.
; Tip Do not run detergent, bleach, or ethanol through the sheath filter.
They can break down the filter paper within the filter body, causing particles
to escape into the sheath fluid, possibly clogging the flow cell.
2 Empty the sheath container and rinse it with DI water.
3 Fill the sheath container with at least 1 L of a 1:10 dilution of bleach or
full-strength BD FACSClean solution.
4 Empty the waste container if needed.
Instrument hardware might be contaminated with biohazardous material.
Use 10% bleach to decontaminate the BD LSR II flow cytometer. Flushing
with 10% bleach is the only procedure recommended by BD Biosciences for
decontaminating the instrument.
LSR2.book Page 103 Tuesday, April 25, 2006 3:34 PM
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