78 BD LSR II User’s Guide
How to Use PI with DNA QC
You can use the PI that comes with the BD DNA QC Particles kit instead of
DAPI. To do so, prepare the CEN and CTN samples as described in the kit
instructions.
CEN Optimization
Use the following procedure to set up BD FACSDiva software for a DNA
experiment that uses DAPI as the DNA-staining dye. If you are using another
sample type, modify the steps accordingly. If you are using PI, substitute PI for
DAPI throughout the procedure.
Preparing the Workspace
1 Choose Instrument > Instrument Configuration and verify the current
configuration.
Figure 3-1 on page 54 shows an Instrument Configuration dialog whose
current configuration is the standard default configuration.
Verify that DAPI appears in the current configuration.
For accurate data results, the Instrument Configuration dialog box must
reflect the physical layout of the BD LSR II octagons and trigons.
Modifications to the current configuration will not apply unless you click
Set Configuration.
2 Choose Edit > User Preferences.
Verify that under the General tab, all checkboxes are deselected (Figure 3-2
on page 55).
Refer to the BD FACSDiva Software Reference Manual for more
information about the instrument configuration and user preferences.
3 Create a new experiment and rename it DNA.
LSR2.book Page 78 Tuesday, April 25, 2006 3:34 PM
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