104 BD LSR II User’s Guide
5 Open the roller clamp by the fluidics interconnect, and drain the fluid into
a beaker for 5 seconds.
6 Remove the DI water tube from the SIP.
7 Prime twice (perform the following twice):
• Press the PRIME button on the fluidics control panel.
• When the STNDBY button lights (amber), press the PRIME button
again.
8 Install a tube with 3 mL of a 1:10 dilution of bleach or full-strength
BD FACSClean solution on the SIP.
9 Press the RUN fluid control button; run the cytometer on HI for 30
minutes.
10 Press the STNDBY fluid control button and depressurize the sheath
container by lifting the vent valve.
11 Repeat steps 2 through 10 with BD FACSRinse solution in place of the
bleach solution.
12 Repeat steps 2 through 10 with DI water in place of the BD FACSRinse
solution.
13 Replace the sheath filter and refill the sheath container with sheath fluid.
LSR2.book Page 104 Tuesday, April 25, 2006 3:34 PM
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