Chapter 4: DNA Analysis 85
6 Adjust the DAPI voltage to place the first peak at approximately channel
50 x 10
3
on the DAPI-A axis.
Figure 4-5 Doublet discrimination with unzoomed plot
7 Use the Zoom-In button to magnify the area showing the singlets and
doublets on the DAPI-A vs DAPI-W plot (Figure 4-6).
Figure 4-6 Doublet discrimination with zoomed plot
If you are unable to distinguish the singlet and doublet populations, see
Chapter 7, Troubleshooting.
doublets
singlets
singlets
doublets
LSR2.book Page 85 Tuesday, April 25, 2006 3:34 PM
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