64 BD LSR II User’s Guide
9 Choose Instrument > Instrument Setup > Calculate Compensation.
If the calculation is successful, a dialog is displayed where you can enter a
name for the compensation setup.
10 Enter a setup name, and click OK.
; Tip To help track compensation setups, include the experiment name,
date, or both in the setup name.
The compensation setup is linked to the MyExperiment instrument
settings, and subsequent acquisitions in MyExperiment are performed with
the new compensation settings.
NOTICE BD Biosciences recommends that you always visually and statistically
inspect automatically calculated overlap values. The means of the positive
controls should be aligned with the means of the negative.
LSR2.book Page 64 Tuesday, April 25, 2006 3:34 PM