Chapter 6: Maintenance 101
Figure 6-1 Sample injection port (SIP)
1 Set the fluid control to RUN.
2 Install a tube containing 3 mL of a bleach solution on the SIP with the
support arm to the side (vacuum on) and let it run for 1 minute.
; Tip For the bleach solution, use BD™ FACSClean solution or a 1:10
dilution of bleach in DI water.
BD FACSClean solution is a bleach-based cleaning agent designed for daily
use in cytometer maintenance.
3 Move the support arm under the tube (vacuum off) and allow the bleach
solution to run for 5 minutes with the event rate set to HI.
4 Repeat steps 2 and 3 with BD™ FACSRinse solution.
BD FACSRinse solution is a detergent-based cleaning solution.
5 Repeat steps 2 and 3 with DI water.
6 Set the fluid control to STNDBY.
tube stop
Bal seal
outer sleeve
tube support arm
sample injection tube
LSR2.book Page 101 Tuesday, April 25, 2006 3:34 PM
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