Chapter 2: Instrument Setup 41
Default Configurations
Each BD LSR II instrument has a default filter and mirror configuration. The
standard default configuration is specified for an instrument with an octagon
detector array for the 488-nm blue laser, and trigon detector arrays for any
optional lasers. See Appendix E for a detailed description of the standard default
configuration, also called the 4-Blue 2-Violet 2-UV 2-Red default configuration.
Additional special-order configurations are specified for instruments with non-
standard detector arrays. For example, there is a 6-Blue 6-Violet 2-UV 3-Red
default configuration for an instrument that has octagon detector arrays for both
the 488-nm blue laser and the 405-nm violet laser, and trigon detector arrays for
both the UV laser and the 633-nm red laser. Detailed descriptions of the
additional special-order configurations are in Appendix F.
BD FACSDiva Instrument Configuration
When you acquire data using BD FACSDiva software, you specify an instrument
configuration. The instrument configuration defines which fluorochrome or
scatter parameter is measured at each instrument detector.
BD FACSDiva software provides a default instrument configuration based on
your BD LSR II. Use the Instrument Configuration dialog to modify, delete, and
create new instrument configurations. (Refer to the Instrument and Acquisition
Controls chapter of the BD FACSDiva Software Reference Manual for details.)
BD LSR II Configuration Upgrade
If you upgrade your BD LSR II cytometer with additional lasers or special order
detector arrays, you will need to run the ICA software to update the
BD FACSDiva database for your instrument. Instructions for installing and using
the ICA software are in Appendix F.
LSR2.book Page 41 Tuesday, April 25, 2006 3:34 PM
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