Chapter 2: Instrument Setup 45
1 Make sure that the flow cytometer is in STNDBY mode.
2 Disconnect the air line (green) from the sheath container (Figure 2-3 on
page 44).
3 Depressurize the sheath container by pulling up on its vent valve.
4 Remove the sheath container lid.
Unscrew the clamp knob and lift.
5 Add 6 L of sheath fluid, such as BD FACSFlow™ solution, to the sheath
container.
NOTICE Do not fill the sheath tank to its maximum capacity (8 L). When
a full tank is pressurized, erratic instrument performance can result.
; Tip For calcium-flux experiments, use a sheath fluid that does not contain
preservatives. For example, use 1X phosphate-buffered saline (PBS).
6 Close the sheath container lid.
7 Reconnect the air line (green).
8 Make sure the lid is tightly sealed with the gasket in place, the clamp knob
is finger-tight, and that the cytometer fluid line (blue) is not kinked.
; Tip Inspect the sheath container periodically, as sheath fluid can cause
corrosion.
LSR2.book Page 45 Tuesday, April 25, 2006 3:34 PM
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