Biotage Isolera Four - Operational Performance Specifications

411829-L, Description and Specifications April 2012 Page 1-18
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1.3.2 Operational Performance Specifications
Cartridge Positions
Isolera Prime, One, and
LS
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Isolera Four 4 (with separate waste channels)
Solvents
Solvent Supply A maximum of 2 x 4 liter (Isolera Prime) or 4 x 4-liter (Isolera One, Four, and LS) non-
glass reservoirs on top of the system (the solvent tray). Larger reservoirs can be placed
on the side of the system. If using glass reservoirs, up to 2.5-liter reservoirs can be
placed on the solvent tray.
Fraction Collector
Fraction Volume Programmable from 1 to 9999 ml (Isolera Prime, One, and Four) or
5 to 99999 ml (Isolera LS), depending on rack type
Max Fraction Number 192 fractions with no rack change (384 with two collection trays), 13x100 mm racks
Max Total Volume 4800 ml with no rack change (9600 ml with two collection trays), 480 ml bottle racks
If using Isolera LS with a funnel rack, the maximum total volume is 320 liter
Rack Types 13x100 mm*, 16x100 mm*, 18x130 mm (for 17.5 x 130 mm test tubes)*,
16x150 mm*, 18x150 mm, 25x150 mm, 120 ml, 240 ml, 480 ml, and funnel rack
†
* Not recommended on Isolera LS.
†
Cannot be used with Isolera One or Isolera Four.
Internal Detector
Wavelength 200 nm to 400 nm (variable), 200 to 800 nm (UV-VIS), or 254 nm (fixed)
The UV-VIS detector is not available with Isolera Prime and the fixed UV detector is not
available with Isolera LS. (Isolera Prime can be upgraded to an Isolera One or Four system.)
Amplitude Range 0 to 6 AU (Absorbance Units); accuracy of ± 0.04 AU
Flow Cell Optical Path 0.3 mm
UV lamp Deuterium discharge, 25 W
Tungsten lamp 10 W (used with the UV-VIS detector)
Pump
Flow Rate Range 1 to 100 ml/min (Isolera Prime), 1 to 200 ml/min (Isolera One and Four), or 50 to 500
ml/min (Isolera LS), in 1 ml/min increments
Pressure Range 0 to 10 bar (0 to 145 psig)
Gradient
Gradient Precision ±3%(Isolera Prime) or ±2%(Isolera One, Four, and LS)
Sample Loading Pump (Isolera LS)
Flow Rate Range 0 to 200 ml/min
Pressure Range 0 to 2 bar (0 to 29 psig)
Display
Screen 10.4" LCD; TFT active matrix; 800 x 600 resolution
Touch Screen Solvent stable