411829-L, Operation April 2012 Page 4-25
4
5. If you extended a finished run or edited a gradient run that was not started or queued up*,
press Gradient to start (or queue up*) the run. Note that if you extend a run on an Isolera
Four system and UV Zero has been performed on another cartridge in between, the system will
perform a UV Zero.
NOTE
When using an Isolera Four system, queued equilibrations take priority
over queued gradient runs.
* Purifications can be queued up when using an Isolera Four system.
4.7.8 Pause, End, or Abort a Purification
WARNING
Keep your hands out of range of the collection arm until it has stopped
moving (with the collection arm in the inner right corner).
The system is pressurized when it is paused.
Pause and Resume a Purification
1. To pause a purification in progress, press Pause in the right-hand panel (in the software). The
collection arm returns to its home position (the inner right corner) and the system is paused.
2. To resume the run from the point at which it was paused, press Resume in the right-hand panel.
End or Abort a Purification
1. Select the Status tab in the right-hand panel.
2. If the purification is in progress:
a. Press Stop. The collection arm returns to its home position (the inner right corner), the
system is paused, and the Stop dialog opens.
b. If you want the system to perform all enabled flushes and line purges, press End.
c. If you do
not want the system to perform the enabled flushes and purges, press Abort. To
release any remaining pressure in the cartridge, press Purge... in the Purge dialog that
opens. (If there are queued runs*, the processing of the queue is started by pressing
Resume in the right-hand panel.)
3. If the purification is not started, i.e. the equilibration is finished but the gradient run is not
started or the run is queued up*, press Remove.
* Purifications can be queued up when using an Isolera Four system.
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