411829-L, Operation April 2012 Page 4-19
4
4.5.2 Run a Purification
WARNING
If you at any time during a purification need to pause the system, press
the Pause button in the right-hand panel (in the software). The collection
arm returns to its home position (the inner right corner) and the system is
paused. Note that the system is pressurized when it is paused. To resume
operation, press the Resume button.
1. Prepare the system as described on page 4-15.
2. Select the Method tab in the right-hand panel.
3. Open or create a new method, see “Create, Open, and Edit Methods” on page 4-2.
4. To run a purification using the displayed method, press Run.... The Run Parameters and
(if estimation is enabled) the Estimation dialog opens. To enable or disable estimation, see
“Enable or Disable Run Requirement Estimation” on page 3-10.
5. In the Run Parameters dialog:
a. Select/press the rack position(s) to be used ( ).
b. If using an Isolera Four system, select/press the cartridge position to be used ( ).
c. If the run is to be performed without equilibration and you want to inject a liquid sample
into the cartridge, you can either use a syringe or the sample loading pump (only
available on Isolera LS). See Table 4-3 on page 4-21 for cartridge loading capacity.
To use the sample loading pump: Press Load Sample and read and follow the
instructions in the Load Sample dialog. When you are done, press Close and connect the
cartridge inlet tubing to the cartridge’s inlet (see Figure 4-10). If you want to reuse the
peristaltic pump tube in the next run (not recommended), clean the tube with methanol
as soon as possible. Note that the sample loading pump can be cleaned while a
purification is in progress. For more information and instructions, see page 5-5.
NOTE
When using an Isolera Four system with automatic rack allocation
enabled, several runs (using the displayed method) can be queued up
simultaneously by selecting two or more cartridge positions in the Run
Parameters dialog.
6. In the Estimation dialog (if present), ensure that a sufficient quantity of the correct solvent is
present in each solvent reservoir, the waste reservoir has sufficient capacity for the run, and the
sufficient number of vessels have been allocated.
7. To start or queue up* the purification, press Equilibrate/Gradient (see Figure 4-15). The
Status tab is selected. Before the run is started, the system performs a UV Zero using the
gradient's initial solvent mix. Note that a gradient run is not started until the UV lamp is
sufficiently warmed up.
8. When the equilibration step (if included) is finished:
a. Load your sample; see “Sample Loading Methods for SNAP and SNAP Ultra Cartridges” on
page 1-4. If a liquid sample is used, press Load Sample... at the Status tab to enable
sample injection. The Load Sample dialog opens. To load a sample using the sample
loading pump (only available on Isolera LS), read and follow the instructions in the dialog.
When you have finished loading the sample, press Close and connect the cartridge inlet
tubing to the cartridge’s inlet (see Figure 4-10). See Table 4-3 on page 4-21 for cartridge
loading capacity.
b. Start or queue up* the gradient run by pressing Gradient at the Status tab.
* Purifications can be queued up when using an Isolera Four system.
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