Biotage Isolera Four - 4.5 Prepare and Run a Purification

411829-L, Operation April 2012 Page 4-7
4
Table 4-1: Rack Selection Table
System with One Collection Tray
Rack Type
Racks
per
Tray
Volume
per Tray
(ml)
Cartridge Type (CV in ml**)
10g*
(15/17)
25g*
(33/45)
50g
(66/85)
100g
(132/164)
75S
§
(264)
340g
(510/582)
75M
§
(528)
750g
§
(990)
75L
§
(1056)
13x100 mm
†
4 432 x 4 2 2/3 4/5 8
16x100 mm
†
4 490 x 4 2 2/3 4/5 7
18x130 mm
†
4 504 x 4 2 2/3 4/5 7
16x150 mm
†
4 770 x 4 2/2 3/3 5
18x150 mm 4 756 x 4 2/2 3/3 5 25x150 mm 4
795 x 4
‡
2/2 3/3 5
120 ml 4 720 x 4 2/2 3/3 5 240 ml 1 4320 2/2 2 480 ml 1 4800 2/2 2
System with Two Collection Trays
¶
Rack Type
Racks
per
Tray
Volume
per Tray
(ml)
Cartridge Type (CV in ml**)
10g*
(15/17)
25g*
(33/45)
50g
(66/85)
100g
(132/164)
75S
(264)
340g
(510/582)
75M
§
(528)
750g
§
(990)
75L
§
(1056)
1500g
#
(1980)
13x100 mm
†
4 432 x 4 2 2/3 4/5 8 16/18 16
16x100 mm
†
4 490 x 4 2 2/3 4/5 7 14/16 15
18x130 mm
†
4 504 x 4 2 2/3 4/5 7 14/15 14
16x150 mm
†
4 770 x 4 2/2 3/3 5 9/10 9
18x150 mm 4 756 x 4 2/2 3/3 5 9/10 10 25x150 mm 4
795 x 4
‡
2/2 3/3 5 9/10 9
120 ml 4 720 x 4 2/2 3/3 5 10/11 10 240 ml 1 4320 2/2 2 3 4 6
480 ml 1 4800
2/2 2 3 3 6
*
Cannot be used on Isolera LS.
†
Not recommended for use on Isolera LS.
‡
When using 53-ml test tubes.
§
Not recommended for use on Isolera Prime.
¶
Not available with Isolera Prime. Two collection trays can be used if the system is upgraded to Isolera One or Four.
#
Not recommended for use on Isolera Prime, Isolera One, or Isolera Four.
**
When two column volumes are listed, the first is for SNAP and the second is for SNAP Ultra.