Possible cause and actionProblem
•
Air in the UV flow cell
Use the Flow restrictor. Use the pH valve instruction
to manually set the Flow restrictor inline (Flow path:pH
valve:Restrictor in-line), or select the Flow restrictor
in the Method Settings phase of a method.
Remove the air by flushing the cell with water or
buffer. If persistent, clean the UV cell. See Section 7.5.1
Clean the UV flow cell, on page 283.
•
Air in buffers
De-gas if necessary.
•
Dirt in the UV flow cell
Clean the UV cell. See Section 7.5.1 Clean the UV flow
cell, on page 283.
•
Dirt in the flow path
Clean the system in accordance to Section 7.6.2 Per-
form System CIP, on page289. Clean the column in ac-
cordance to Section 7.6.3 Perform Column CIP, on
page 297.
Ghost peaks
•
Contact Service
The UV lamp is broken
or worn out
370 ÄKTA avant User Manual 29035184 AE
8 Troubleshooting
8.3 Troubleshooting: Monitors
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