Possible cause and actionProblem
•
Quick scan has not detected the correct Cassette
Clean the Cassette type code of the Cassette. See
Section 7.6.4 Clean built-in fraction collector, on
page 301.
•
Unsupported deep well plate is used.
Make sure that approved deep well plates are used.
See Section 3.10.3 Cassettes, cassette tray and racks,
on page 89.
•
Wells in deep well plates are prefilled to a volume
above 25% of the total well volume
Full scan will not work with prefilled wells during these
conditions.
•
The deep well plate is not correctly positioned in
the Cassette
See Section 5.8.1 Prepare the built-in fraction collector,
on page 203 for information of how to place the deep
well plate
•
Dirt on the nozzle or DropSync sensor diode win-
dows. Dirt may effect where the drops fall.
Clean nozzle and DropSync sensor diode windows.
See Section 7.6.4 Clean built-in fraction collector, on
page 301.
The Fraction collector
fractionates in the
wrong well or tube
•
Some parts in a method require that the Frac
drawer is closed.
Do not open the Frac drawer when the symbol indicat-
ing ongoing fractionation is displayed on the Instru-
ment display (see Buttons and indicators, on page41).
Error message when
the Frac drawer is
opened during a run
•
The Detector - Outlet valve delay volume has been
set to zero.
1
In System Control, select System:Settings:Frac-
tion collections:Delay volumes.
2
Correct the Detector - Outlet valve delay volume.
The instrument does
not fractionate via the
Outlet valve ports
392 ÄKTA avant User Manual 29035184 AE
8 Troubleshooting
8.5 Troubleshooting: Fraction collector
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