Possible cause and actionProblem
Action: End a manual run before starting a method run.When starting a
method run during a
manual run, the Frac-
tion collector gener-
ates an error message
indicating that the
wrong Cassettes are in
place, even though the
correct Cassettes are
present in the Fraction
collector.
Action:High pressure alarm
when collecting frac-
tions with the Fraction
collector
•
Decrease the flow rate or use Outlet valve fractiona-
tion,
or
•
(only if ÄKTA avant 25 is used) replace tubing accord-
ing to instructions below.
Replace the tubing between Column valve and Outlet
valve by tubing with i.d. 0.75 mm (tubing: 6, 7, 8, 9, 1R
and 2R).
Note:
Delay volumes in System Settings have to be changed
to:
Detector - Frac: 635 μl
Detector - Outlet valve: 260 μl
Restrictor volume: 85 μl
To change the Fraction collector capillary from i.d. 0.5
to i.d. 0.75 mm, contact GE Service.
Note:
If the Fraction collector capillary is also changed, the
delay volume, Detector - Frac, has to be changed to
950 μl.
Action: If possible, place the Cassettes close to waste, i.e.,
position 5 or 6 during fractionation.
Spillage in Fraction
collector when the Frac
arm is moving from
Cassette placed in posi-
tion 1 and 2 to waste
ÄKTA avant User Manual 29035184 AE 393
8 Troubleshooting
8.5 Troubleshooting: Fraction collector
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