Instructions
Follow the instructions below to perform a Flow cell path length test and set a new cell
length.
ActionStep
Flush the UV flow cell with demineralized water using the Pump, and then
leave the UV flow cell filled with water.
1
Tap Flow cell path length.2
Result: The following screen opens.
Set the Cell value to 2.00 mm by using the up/down arrows or enter the
value in the text box.
3
Flush the UV flow cell thoroughly and leave it filled with demineralized water.4
Tap Set baseline to capture a new reference value for the test.
Result: Abs should show a value close to 0 mAU.
Flush the UV flow cell with 1.0% acetone solution, and then leave it filled.5
Note the new Abs value.
Calculate the actual Flow cell path length by using the following formula:6
Cell (mm) = 2.00 * (new Abs value / 340 )
ÄKTA start Maintenance Manual 29060308 AD 45
3 Maintenance operations
3.5 UV Monitor
3.5.3 Flow cell path length
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