3.5.2 Diagnostics
Description
Diagnostics is used to conduct a Dark current test and/or a Stray light test to assess
electrical noise or presence of stray light.
DescriptionTest
•
Switches off the UV LED during the test.
•
The test result is presented as Pass if the Signal re-
sponse is below 50 mV.
Dark current test
•
Checks the absorbance when the UV flow cell is filled
with 15% acetone.
•
Absorbance (Abs) should have a value > 2000 mAU.
Stray light test
Check for electrical noise
Follow the instructions below to perform a Dark current test.
ActionStep
Make sure that:1
•
The UV flow cell is assembled tightly.
•
The inlet and the outlet tubing, and connectors are tightened to block
any stray light from entering.
•
The system is not exposed to direct sunlight.
Flush and fill the UV flow cell with demineralized water using a syringe. Make
sure that there are no air bubbles in the syringe.
2
ÄKTA start Maintenance Manual 29060308 AD 41
3 Maintenance operations
3.5 UV Monitor
3.5.2 Diagnostics