4.2.8 Configuration of UV monitors
Using two UV monitors
The UV monitor U9-L module can be used in two configurations, UV monitor U9-L and UV monitor U9-
L, 2nd. The configuration is defined by the module's Node ID.
It is possible to use two UV monitors in ÄKTA pure, in the following combinations:
•
UV monitor U9-M together with UV monitor U9-L, 2nd
•
UV monitor U9-L together with UV monitor U9-L, 2nd
UV monitor U9-L, 2nd can be located anywhere in the flow path and is therefore shown in the Process
Picture as a component without a fixed place. This means that it is possible to place U9-L, 2nd before
the other UV monitor in the flow path.
If U9-L, 2nd is placed on the high pressure side of the column, pressure limits have to be con-
sidered. See UV monitor options, on page 416 for pressure limits.
Note:
System properties
Follow the instruction below to update the system properties.
ActionStep
Open the system properties Edit dialog.1
Select Monitors and sensors from the Component types list.2
Select components and properties according to the following table:3
PropertyComponent selectionMonitor
U9-L (fixed)UV monitorUV monitor U9-L
V9-M (variable)UV monitorUV monitor U9-M
N/AUV monitor 2nd (U9-L)UV monitor U9-L, 2nd
System settings
If the monitor is not placed in the recommended position using the standard tubing kit, the delay volume
must be set. See Check/Set delay volume, on page 146.
For UV monitor U9-L the flow cell length must be set. This is done as a calibration. See Perform the
calibration, on page 313.
140 ÄKTA pure User Manual 29119969 AB
4 System configuration
4.2 Configure modules
4.2.8 Configuration of UV monitors
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