5.7.3 Sample application using a sample loop
Introduction
A sample loop is recommended for injection of smaller sample volumes onto the column.
A sample loop can be connected to either the Injection valve or the Loop valve. When using the Loop
valve, up to five loops can be connected simultaneously.
Sample loop is called capillary loop in UNICORN.
Note:
How to fill a sample loop
Follow the instructions below to fill the sample loop with sample.
ActionStep
Connect a suitable sample loop to Injection valve ports LoopF (fill) and LoopE (empty).1
Fill a syringe with sample.2
Connect the syringe to the Injection valve port Syr.3
190 ÄKTA pure User Manual 29119969 AB
5 Operation
5.7 Sample application
5.7.3 Sample application using a sample loop