9.5.1 General information about biocompatibility and chemical resistance
Biocompatibility
The ÄKTA pure instrument is designed for maximum biocompatibility, with biochemically inert flow
paths constructed mainly from titanium, PEEK and highly resistant fluoropolymers and fluoroelastomers.
Titanium is used as far as possible to minimize contribution of potentially deactivating metal ions such
as iron, nickel and chromium. There is no standard stainless steel in the flow path. Plastics and rubber
materials are selected to avoid leakage of monomers, plasticizers or other additives.
Cleaning chemicals
Strong cleaning works well with 2 M sodium hydroxide, 70% acetic acid or the alcohols methanol,
ethanol and isopropyl alcohol. Complete system cleaning using 1 M hydrochloric acid should be
avoided in order to not damage the pressure sensors. If you are cleaning separation media using 1 M
hydrochloric acid, use loop injections of the acid and make sure that the column is not mounted on
the Column Valve V9-C. The Column Valve V9-C contains a pressure sensor which can be damaged
by 1 M hydrochloric acid.
If sodium hypochlorite is used as sanitizing agent instead of 2 M sodium hydroxide, use a concentration
up to 10%.
Organic solvents
Reversed phase chromatography of proteins works well with 100% acetonitrile and additives trifluo-
roacetic acid (TFA) up to 0.2% or formic acid up to 5%.
Strong organic solvents like ethyl acetate, 100% acetone or chlorinated organic solvents should be
avoided. These might cause swelling of plastic material and reduce the pressure tolerance of PEEK
tubing. For this reason, flash chromatography and straight (“normal”) phase chromatography is gen-
erally not recommended on the system
Assumptions made
The ratings are based on the following assumptions:
•
Synergy effects of chemical mixtures have not been taken into account.
•
Room temperature and limited overpressure is assumed.
Chemical influences are time and pressure dependent. Unless otherwise stated, all concentra-
tions are 100%.
Note:
432 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.5 Chemical resistance guide
9.5.1 General information about biocompatibility and chemical resistance
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