2.4.1 System pumps
Introduction
This section describes the design and main functions of the system pumps, and also the pump piston
rinsing systems. The system can also be equipped with an external, optional sample pump, see Sec-
tion 3.4 Sample pump S9 and S9H, on page 108.
Function of the system pumps
The ÄKTA pure instrument is fitted with two high precision system pumps, System pump A and System
pump B. The system pumps can be used individually, or in combination to generate isocratic or gradient
elution in purification methods.
Each pump module consists of two pump heads that work alternately to give a continuous, low pulsation,
liquid delivery. To ensure delivery of correct liquid volume, the pumps must be free from air. Each pump
head is equipped with a purge valve that is used for this purpose. See Section 5.4 Prime inlets and purge
pump heads, on page 161.
The table below contains the operating limits and labels of the system pumps of ÄKTA pure 25 and
ÄKTA pure 150, respectively.
Max. pressureFlow ratePump typeLabelConfiguration
20 MPa0.001 – 25 ml/min
Note:
When running the
Column packing
flow instruction, the
maximum flow rate
is 50 ml/min.
P9P9 A and P9 BÄKTA pure 25
5 MPa0.01 – 150 ml/min
Note:
When running the
Column packing
flow instruction, the
maximum flow rate
is 300 ml/min.
P9HP9H A and P9H BÄKTA pure 150
34 ÄKTA pure User Manual 29119969 AB
2 The ÄKTA pure instrument
2.4 Instrument modules
2.4.1 System pumps
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