9.9.4 Manual instructions - Fraction collection
The table below describes the fraction collection related manual instructions available for ÄKTA pure.
DescriptionInstruction name
Fractionation is used when collecting fractions with a fraction
collector.
Fractionation
Stop fractionation ends the fractionation after the set delay vol-
ume (specified in System Settings:Tubing and Delay volumes)
has been collected. The outlet valve is then turned to position
Waste.
Note:
If Stop fractionation is issued when both Fractionation and Peak
fractionation are active, fractionation is stopped after the set delay
volume has been collected. The outlet valve remains in position
Frac and peak fractionation continues.
Stop fractionation
Peak fractionation enables collection of only those peaks that
fulfill the conditions set in the Peak fractionation parameters in-
struction.
Peak fractionation
Stop peak fractionation ends the peak fractionation after the set
delay volume (specified in System Settings:Tubing and Delay
volumes) has been collected. The outlet valve is then turned to
position Waste.
Stop peak fractionation
Only for Fraction collector F9-C. Last tube filled sets the action
to perform after the fraction collector fills the last tube of the run:
pause the fractionation, direct the flow to one of the outlet ports
of the outlet valve or direct the flow to waste.
Last tube filled
Only for Fraction collector F9-R. Sets fraction numbers to restart
from 1. The restart occurs when the instruction is issued. The in-
struction overrides the continuous numbering mode if Fractiona-
tion numbering mode is set to Continue in System Settings.
Reset frac number
Fraction collector F9-C: Feed tube moves the fractionation arm
to the position specified by the parameter Start position, after
the set delay volume has been collected. A fraction mark is given
in the chromatogram.
Feed tube
Fraction collector F9-R: Feed tube moves the tube rack forward
one tube after the set delay volume has been collected and a
fraction mark is set. When fractionation or peak fractionation is
not ongoing, Feed tube moves the rack instantly and no fraction
mark is set.
474 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.9 Manual instructions
9.9.4 Manual instructions - Fraction collection
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