6.10 System Test UV U9-M (variable)
Method description
The System test with UV monitor U9-M checks the functionality of the solvent delivery, the pumps, the
System pump pressure sensor, UV monitor U9-M and the Conductivity monitor.
The method run takes approximately 35 minutes.
Required configuration
A correctly installed UV monitor U9-M and a correctly installed Conductivity monitor are required to
run the test.
Required material
The following materials are required:
•
Distilled water
•
1% acetone and 1.00 M NaCl in distilled water
•
Ref 1 tubing, see Reference capillary, on page 426.
•
Syringe, 25-30 ml
•
Mixer, 1.4 ml
Prepare the test
Follow the instructions below to prepare the system before method start.
ActionStep
Direct outlet tubing W and W1 to a waste container.1
Place inlet tubing A1 into distilled water.2
Place inlet tubing B1 into a solution of 1% acetone and 1.00 M NaCl in distilled water.3
Connect the Ref 1 tubing between Injection valve port Col and the UV monitor U9-M inlet,
to generate a back pressure.
4
Make sure that the Mixer with a chamber volume of 1.4 ml is installed.5
For further information, see Section 7.8.3 Replace the Mixer, on page 322.
Prime the buffer inlets and purge System pump A and System pump B. See Section 5.4.1
System pumps, on page 162.
6
If the test is performed at cold room temperature, select Conductivity:Cond temp compensa-
tion in the System Settings dialog, and set the Compensation factor to 2.1.
Note:
248 ÄKTA pure User Manual 29119969 AB
6 Performance tests
6.10 System Test UV U9-M (variable)
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