9.3 Tubing and connectors
Tubing types
The table below shows the tubing types used in ÄKTA pure.
Volume/cmScope of useColorDescription
0.5 μlHigh pressure tubing
Reference capillary 1
Tubing Kit 0.25
BluePEEK, o.d. 1/16", i.d. 0.25
mm
2.0 μlHigh pressure tubing
Tubing kit 0.5 (stan-
dard)
OrangePEEK, o.d. 1/16", i.d. 0.50
mm
4.4 μlHigh pressure tubing
Tubing kit 0.75
GreenPEEK, o.d. 1/16", i.d. 0.75
mm
7.8 μlHigh pressure tubing
Tubing kit 1.0
BeigePEEK, o.d. 1/16", i.d. 1.0 mm
20.0 μlInlet tubingTransparentFEP, o.d. 1/8", i.d. 1.6 mm
66.0 μlInlet tubing for high
flow rate and high vis-
cosity
TransparentFEP, o.d. 3/16", i.d. 2.9 mm
4.4 μlNarrow inlet tubing
(optional)
TransparentETFE, o.d. 1/16", i.d. 0.75
mm
7.8 μlOutlet and waste tub-
ing
TransparentETFE, o.d. 1/16", i.d. 1.0 mm
0.3 mlWaste tubing from
Buffer tray
TransparentSilicone, o.d. 12 mm, i.d. 8
mm
Pump rinse solution
tubing
Note: •
Many different sizes/types of tubing can be connected to a chromatography system. Tubing
with a smaller inner diameter (i.d.) holds less delay volume and will therefore generate less
dilution of the protein peak. Narrow tubing, however, increases the system pressure, espe-
cially when running at high flow rates. The tubing used should match the application needs.
See Section 9.4 Recommended tubing kits for prepacked columns, on page 428 for more
information.
•
When using the high pressure tubing kit with i.d. 1.0 mm to allow high flow rates in combi-
nation with high viscosities in the pumps, inlet tubing with a larger i.d. than standard might
be needed to avoid outgassing.
420 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.3 Tubing and connectors
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