GE AKTApure - Create a Method

5.9.1 Create a method
Introduction
The predefined methods are built up using phases, where each phase corresponds to a step in a
chromatography run with a number of properties associated with that phase.
See UNICORN Method Manual for more information about method structure, definitions and concepts
of methods in UNICORN.
Predefined methods
There are several predefined methods to choose from. All the predefined methods are listed below.
The predefined methods available for each system are defined by the Instrument Configuration. Refer
to Section 9.7 Predefined methods and phases, on page 438 for more information about each method.
Purification methods
•
Affinity Chromatography (AC)
•
Affinity Chromatography (AC) with Tag Removal
•
Anion Exchange Chromatography (AIEX)
•
Cation Exchange Chromatography (CIEX)
•
Chromatofocusing (CF)
•
Desalting
•
Gel filtration (GF)
•
Hydrophobic Interaction Chromatography (HIC)
•
Manual Loop Fill
•
NHS-coupling
•
Reversed Phase Chromatography (RPC)
Maintenance methods
•
Column CIP
•
Column Performance Test
•
Column Preparation
•
System CIP
•
System Preparation
Main steps when defining a new method
The main steps when defining a method are:
1
Create/open a method
•
Create a Predefined method (including a set of phases that may be edited)
212 ÄKTA pure User Manual 29119969 AB
5 Operation
5.9 Create a method and perform a run
5.9.1 Create a method