DescriptionPart
Inlet valve4
System pump B5
System pump A6
Pressure monitor7
Mixer8
Injection valve9
Sample loop or Superloop10
Column valve11
Column12
UV monitor13
Conductivity monitor14
Flow restrictor15
Outlet valve16
Fraction collector17
WasteW, W1, W2
Select Mixer chamber
To obtain a homogeneous buffer composition, it is important to use a Mixer chamber suitable for the
flow rate of the method. The tables below show what Mixer chambers to use in ÄKTA pure at different
flow rates.
If the liquids are difficult to mix, use a larger Mixer chamber to achieve optimal mixing. However, note
that a larger Mixer chamber distorts and delays the gradient.
The default mixer size is 1.4 ml for both systems. ÄKTA pure 150 also includes a 5 ml mixer. Other
mixer sizes are available as accessories.
Flow rate [ml/min], Binary gradientMixer chamber volume [ml]
ÄKTA pure 150ÄKTA pure 25
Not recommended0.1-50.6
0.5-150.5-151.4
2-502-255
15-150Not compatible15
152 ÄKTA pure User Manual 29119969 AB
5 Operation
5.2 Prepare the flow path
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