GE AKTApure - Page 182

Then...When preparing to inject...
•
in the Method editor, make the following selections for the
Sample Application phase of the method to be run:
-
select Inject sample directly onto column, and
-
select Inject all sample using air sensor.
•
manually prime the sample inlet tubing with sample or buffer,
see Prime sample inlets, on page 168, and make sure that the
tubing is immersed in sample before starting the run.
•
make sure that the flow path from the sample inlet valve up to
the injection valve is filled with sample or an appropriate buffer
and that the buffer inlet tubing is immersed in buffer.
all the sample
Maximize precision and accuracy
To achieve full precision and accuracy when a volume of sample is injected directly onto the column,
make the following selections in the Sample Application phase of the method to be run (refer to Sec-
tion 5.9 Create a method and perform a run, on page211 for more information on methods and phases):
•
select Inject sample directly onto the column,
•
select Inject fixed sample volume and set the volume to be injected,
•
enable the function Prime sample inlet with and set the volume to be used for priming.
Result: the step Finalize sample injection is automatically deactivated in order to maximize precision
and accuracy. See Minimize sample loss, on page 181 for more information.
If manual priming of the flow path up to the injection valve is preferred, enable Prime
sample inlet with but set the volume to 0 ml.
Note:
•
If the system pump is used to load the sample: enable the function Wash sample flow path with
buffer after sample application in order to ensure that the correct sample volume is loaded onto
the column.
182 ÄKTA pure User Manual 29119969 AB
5 Operation
5.7 Sample application
5.7.1 Sample application using direct injection onto the column