ActionStep
In the Manual instructions dialog:1
•
Select Flow path:Injection valve.
•
Select Sample pump load from the Position drop-down list.
•
Click .
Result: The Injection valve switches to Sample pump load position.
Make sure that the sample inlet tubing from the sample vessel is connected to the Sample
inlet valve.
2
In the Manual instructions dialog:3
•
Select Alarms:Alarm sample pressure.
•
Set Mode as Enabled.
•
Set a High alarm level that is below the maximum pressure of the Superloop.
•
Click .
In the Manual instructions dialog:4
•
Select Pumps and pressures:Sample flow.
•
Set Flow rate to an appropriate value for the Superloop size, in this example 10 ml/min.
•
Click .
Result: A sample flow starts, in this example of 10 ml/min.
When the Superloop is filled with as much volume as is needed, click the End icon in the
System Control toolbar to end the run.
5
Plug the Syr port on the Injection valve with a Stop plug.6
186 ÄKTA pure User Manual 29119969 AB
5 Operation
5.7 Sample application
5.7.2 Sample application using a Superloop
™
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