Illustration of fractions and fraction marking using peak fractionation
The illustration below shows the fractions collected, and the numerical marking of fractions, when
peak fractionation is used with Fraction collector F9-R.
When peak fractionation is used, Fraction collector F9-R collects the delay volumes in the fractions
preceeding each peak. In the chromatogram above, delay volumes are collected in fractions 1, 5 and
8. The numerical fraction marks for the delay volume fractions are not indicated on the chromatogram.
Fraction collector F9-C does not save the delay volumes as fractions, instead the delay volumes are
disposed of in the waste funnel.
If two fraction collectors are used, the fractions collected by the second Fraction collector are
indicated by the prefix 2: (i.e. fractions 2:1, 2:2, 2:3 etc.).
Note:
Fractionation modes for Fraction collector F9-C
There are three fractionation modes for Fraction collector F9-C, Automatic, Accumulator and DropSync.
Spillage between collection vessels during a run is avoided with all three fractionation modes.
•
Automatic: The fraction collector uses the Drop Sync mode for flow rates up to 2 ml/min and auto-
matically switches to Accumulator mode for higher flow rates.
•
Accumulator: Liquid is collected during movement between tubes or wells. The liquid is then dis-
pensed in the next well or tube. Fractionation with accumulator can be used at all flow rates.
•
DropSync: When using DropSync , the sensors in the Dispenser head detect when a drop is released
from the nozzle. The Dispenser head moves to the next well or tube just after a drop is released.
Fractionation with DropSync can be used at flow rates up to 2 ml/min. If only one Cassette is used
and it is placed near the waste funnel, DropSync can be used at higher flow rates. Volatile solutions
and solutions with low surface tension may require a lower flow.
Fractionation settings for Fraction collector F9-R
There are two fractionation settings for Fraction collector F9-R, DropSync off or DropSync on .
Dropsync off: No synchronization of collection.
ÄKTA pure User Manual 29119969 AB 209
5 Operation
5.8 Fractionation
5.8.3 Fractionation overview
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