Actions in the process picture pane
It is possible to manually interact with an ongoing method through the Process Picture, see UNICORN
System Control Manual.
Using UNICORN to control the pressure during a
run
The function Pressure control allows a method to be run with the set flow rate without the risk of a
method stop due to pressure alarm. Pressure control is enabled in the instructions System flow and
Sample flow. Refer to Section 9.15 Pressure control, on page 501 for more information.
The system configuration affects the pressure
Using narrow tubing between components will improve resolution but will lead to increased back
pressure in the system. Narrow tubing after the column will increase the pressure in the column at a
given flow rate. Make sure that the sensor limits for the pre-column pressure are set so that the maxi-
mum pressure for the column in use is not exceeded.
If Column valve V9-C or V9H-C is mounted, the integrated pressure sensor of the valve allows
the system to monitor the post-column pressure. The limit for the pressure sensor in Column
valve V9-C or V9H-C is automatically set so that the UV monitor and the pH monitor are pro-
tected from high pressure. If Column valve V9-C or V9H-C is not mounted, make sure to keep
the pressure in the system after the column below the pressure limits for the modules in the
flow path.
Note:
220 ÄKTA pure User Manual 29119969 AB
5 Operation
5.9 Create a method and perform a run
5.9.3 Monitor a run
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