Possible causes of a failed test
The table below describes possible causes of a failed test. When possible sources of error have been
checked and corrected, repeat the test.
ActionCause
Air in pumps: Make sure to prime inlet tubing A1 and purge System
pump A before method start, see Section 5.4.1 System pumps, on
page 162.
Incorrect volumes collected
in the tubes, and distur-
bances of system pressure
curves:
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on page 387.
•
Air trapped in System
pump A
•
Faulty System pump A
Make sure that the system was correctly prepared, see Prepare the
test, on page 237.
Incorrect preparation of
buffer and tubing
ÄKTA pure User Manual 29119969 AB 239
6 Performance tests
6.6 Fraction collector F9-C test
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