GE AKTApure - Page 241

Evaluate the result
Check that the correct volumes have been collected in the tubes. The tubes should contain the following:
•
Tube 1: The delay volume
•
Tube 2-4: 2 ml
•
Tube 5-6: 1 ml
Also, check that the fractionation marks in the chromatogram correspond to the filled tubes and that
spillages are kept to a minimum.
For further information on delay volumes and fractionation marks, see Delay volume, on page 208.
Possible causes of a failed test
The table below describes possible causes of a failed test. When possible sources of error have been
checked and corrected, repeat the test.
ActionCause
Air in pumps: Make sure to prime inlet tubing A1 and purge System
pump A before method start, see Section 5.4.1 System pumps, on
page 162.
Incorrect volumes collected
in the tubes, and distur-
bances of system pressure
curves:
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on page 387.
•
Air trapped in System
pump A
•
Faulty System pump A
Make sure the fraction collector delivery arm is positioned above
tube number 1 before starting the test.
Liquid collected in wrong
tubes:
Make sure that the system was correctly prepared, see Prepare the
test, on page 240.
Incorrect preparation of
buffer and tubing
ÄKTA pure User Manual 29119969 AB 241
6 Performance tests
6.7 Fraction Collector F9-R Test