Run and evaluate the test
Follow the instructions described in Section 6.1 General performance test actions, on page226 to start,
run and automatically evaluate the performance test.
Possible causes of a failed test
The table below describes possible causes of a failed test. When possible sources of error have been
checked and corrected, repeat the test.
ActionCause
Air in pump: Make sure to prime inlet tubing Buffer and purge sample
pump before method start, see Section 5.4.2 Sample pump, on
page 168.
The two injected peaks of liq-
uid are not delivered as pro-
portional peaks:
•
Air trapped in Sample
pump
•
Faulty Sample pump
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on page 387.
Air in pump: Make sure to prime inlet tubing Buffer and purge sample
pump before method start, see Section 5.4.2 Sample pump, on
page 168.
The delivery of liquid is pulsat-
ing (unstable pump pressure)
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on page 387.
Folded, twisted or blocked tubing: Check the tubing.The pump pressure is too
high (at a flow rate of 25
ml/min or 150 ml/min for
ÄKTA pure 25 and ÄKTA pure
150, respectively)
The Sample pump pressure monitor is not calibrated: Calibrate the
pressure monitor, see Section 7.7.2 Calibrate the pressure monitors,
on page 305.
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on page 387.
Make sure that the system was correctly prepared, see Prepare the
test, on page 242.
Incorrect preparation of
buffer and tubing
ÄKTA pure User Manual 29119969 AB 243
6 Performance tests
6.8 Sample pump tests
To Next Page
Loading...
