GE AKTApure - Page 246

Faulty Step Response Result
ActionCause
Air in pumps: Make sure to prime the buffer inlets and to purge the
System pumps before method start. See Section 5.4.1 System pumps,
on page 162.
If all values are faulty:
•
Air in the pump or a faulty
pump
Faulty pump: See Chapter 7 Maintenance, on page 252.
Replace piston seals. See Chapter 7 Maintenance, on page 252.Faulty values at 5%:
•
Damaged pump piston
seal in System pump B
Replace piston seals. See Chapter 7 Maintenance, on page 252.Faulty values at 95%:
•
Damaged pump piston
seal in System pump A
Faulty UV Absorbance Test
ActionCause
Make sure that the acetone solution is 1% and that no solution has
evaporated.
Incorrectly prepared acetone
solution
See, Calibration of the UV monitor U9-L flow cell length, on page 312.Wrong UV cell path length
set in UNICORN
Faulty Pulsation Test
ActionCause
Make sure to prime and purge the system pumps before starting
the test, see Section 5.4.1 System pumps, on page 162.
Air trapped in the pumps
Faulty Conductivity Test Result
ActionCause
See Conductivity monitor, on page 367.Faulty Conductivity monitor
Make sure the NaCl solution is 1.00 M.Incorrectly prepared NaCl
solution
If the test is performed at cold room temperature, open the System
Settings dialog, select Conductivity:Cond temp compensation and
set the Compensation factor to 2.1
The value set for the Cond
temp compensation factor is
not optimal
246 ÄKTA pure User Manual 29119969 AB
6 Performance tests
6.9 System Test UV U9-L (fixed)