Faulty Step Response Result
ActionCause
Air in pumps: Make sure to prime the buffer inlets and to purge the
System pumps before method start. See Section 5.4.1 System pumps,
on page 162.
If all values are faulty:
•
Air in the pump or a faulty
pump
Faulty pump: See Chapter 7 Maintenance, on page 252
Replace piston seals. See Chapter 7 Maintenance, on page 252.Faulty values at 5%:
•
Damaged pump piston
seal in System pump B
Replace piston seals. See Chapter 7 Maintenance, on page 252.Faulty values at 95%:
•
Damaged pump piston
seal in System pump A
Faulty UV Response Test Result
ActionCause
Restart the instrument to calibrate the UV monitor.Faulty UV monitor
See Section 8.3 Troubleshooting: Monitors, on page 360.
Faulty UV Absorbance Test
ActionCause
Make sure that the acetone solution is 1% and that no solution has
evaporated.
Incorrectly prepared acetone
solution
Faulty Pulsation Test
ActionCause
Make sure to prime and purge the system pumps before starting
the test, see Section 5.4.1 System pumps, on page 162.
Air trapped in the pumps
250 ÄKTA pure User Manual 29119969 AB
6 Performance tests
6.10 System Test UV U9-M (variable)